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Article

ThermoAlign: a genome-aware primer design tool for tiled amplicon resequencing.

Scientific reports - 16 Mar 2017

Francis Felix, Dumas Michael D, Wisser Randall J

Abstract excerpt

Isolating and sequencing specific regions in a genome is a cornerstone of molecular biology. This has been facilitated by computationally encoding the thermodynamics of DNA hybridization for automated design of hybridization and priming oligonucleotides. However, the repetitive composition of genomes challenges the identification of target-specific oligonucleotides, which limits genetics and genomics research on...

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Identifiers

PMID: 28300202
DOI: 10.1038/srep44437
PMCID: PMC5353602

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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