Article

A humanized nanobody phage display library yields potent binders of SARS CoV-2 spike

2021-10-24

Abstract excerpt

Neutralizing antibodies targeting the SARS-CoV-2 spike protein have shown a great preventative/therapeutic potential. Here, we report a rapid and efficient strategy for the development and design of SARS-CoV-2 neutralizing humanized nanobody constructs with sub-nanomolar affinities and nanomolar potencies. CryoEM-based structural analysis of the nanobodies in complex with spike revealed two distinct binding modes....

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: f9ded703-81bd-5e71-a9a8-a48f25a93511
DOI: 10.1101/2021.10.22.465476

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A humanized nanobody phage display library yields potent binders of SARS CoV-2 spikeDOI 10.1101/2021.10.22.465476Read

A humanized nanobody phage display library yields potent binders of SARS CoV-2 spikeSemantic proximity Read

Nanobody Repertoires for Exposing Vulnerabilities of SARS-CoV-2Semantic proximity Read

Nanobodies to multiple spike variants and inhalation of nanobody-containing aerosols neutralize SARS-CoV-2 in cell culture and hamstersSemantic proximity Read

Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2Semantic proximity Read

A panel of nanobodies recognizing conserved hidden clefts of all SARS-CoV-2 spike variants including OmicronSemantic proximity Read

Peptide derived nanobody inhibits entry of SARS-CoV-2 variantsSemantic proximity Read

A potent neutralizing nanobody against SARS-CoV-2 with inhaled delivery potentialSemantic proximity Read

Potent neutralizing nanobodies resist convergent circulating variants of SARS-CoV-2 by targeting diverse and conserved epitopes.Semantic proximity Read

Biparatopic sybody constructs neutralize SARS-CoV-2 variants of concern and mitigate emergence of drug resistanceSemantic proximity Read

Highly synergistic combinations of nanobodies that target SARS-CoV-2 and are resistant to escapeSemantic proximity Read

Potent neutralization of clinical isolates of SARS-CoV-2 D614 and G614 variants by a monomeric, sub-nanomolar affinity NanobodySemantic proximity Read

Structure-guided multivalent nanobodies block SARS-CoV-2 infection and suppress mutational escape.Semantic proximity Read