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Low-temperature HILIC provides enhanced separations and stability for LC-MS-based metabolomics

2025-12-22

Abstract excerpt

<h4> Abstract </h4> Liquid chromatography–mass spectrometry is a potent and robust tool for studying metabolism. However, conventional workflows can suffer from poor peak shapes, limited pressure tolerance, co-elution of polar metabolites, and unstable retention times. Here, we describe the development of a more stable HILIC method for LC-MS metabolomics of human plasma and cell extracts, optimizing a zwitterion...

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Literature Corpus work
f5bca340-70e2-52ec-bcd9-b6dcb93fd592
DOI
10.64898/2025.12.19.695573
Open publication

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