Article

Peptide Identification and Quantification by Gas Phase Fractionation Enables Proteomics Without Liquid Chromatography

2020-05-27

Abstract excerpt

Liquid chromatography mass spectrometry (LC-MS) delivers sensitive peptide analysis for proteomics, but the methodology requires extensive analysis time, hampering throughput. Here, we demonstrate that flow injection analysis data-independent acquisition (FIA-DIA), using gas-phase peptide separation instead of LC, offers extremely fast proteome analysis. Incorporating ion mobility with FIA-DIA, we demonstrate...

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Literature Corpus work: 504f3189-42f5-58e3-95f6-120780fe7214
DOI: 10.26434/chemrxiv.12367376.v1

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