Article

Rapid Histone Post-Translational Modification Analysis Using Alternative Proteases and Tandem Mass Tags

2026-02-15

Abstract excerpt

Histone post-translational modifications (PTMs) alter chromatin dynamics and contribute to the regulation of gene expression in health and disease, yet mass spectrometry-based histone PTM analysis remains constrained by inefficient sample preparation workflows. Here, we develop RIPUP ( R apid I dentification of histone P TMs in U nderivatized P eptides), a streamlined multi-protease workflow that reduces samp...

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Literature Corpus work: 9c8b53b5-85af-5e45-ad59-aca79436d9d5
DOI: 10.64898/2026.02.13.705817

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