Article

Combining Cas9 and dCas9 facilitates genome editing in genes associated with viability or welfare issues, or within paralogous gene clusters

2026-05-07

Abstract excerpt

The high efficiency of genome editing presents a challenge when modifying genes associated with viability, welfare, or fertility issues, as implementation of the technology frequently results in mosaic animals with bi-allelic mutations. Combining deactivated Cas9 (dCas9) with Cas9 has been proposed as a strategy to protect one of the two target alleles from editing. We piloted this strategy with 11 genes that are...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: f40f0359-fb80-5f28-9631-da74ee34c54a
DOI: 10.64898/2026.05.05.721005

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Combining Cas9 and dCas9 facilitates genome editing in genes associated with viability or welfare issues, or within paralogous gene clustersDOI 10.64898/2026.05.05.721005Read

Evaluation of mutation rates, mosaicism and off target mutations when injecting Cas9 mRNA or protein for genome editing of bovine embryos.Semantic proximity Read

Gene editing in human development: ethical concerns and practical applicationsSemantic proximity Read

Germ line genome editing in clinics: the approaches, objectives and global societySemantic proximity Read

Molecular breeding of farm animals through gene editingSemantic proximity Read

Promoting Cas9 degradation reduces mosaic mutations in non-human primate embryos.Semantic proximity Read

Comparison of the efficiency and precision of Base editor and CRISPR/Cas9 for inducing defined point mutation (S395F) in ovine embryos.Semantic proximity Read

Efficient CRISPR-Cas9-mediated genome editing of the cane toad ( <i>Rhinella marina</i> )Semantic proximity Read

A non-inheritable maternal Cas9-based multiple-gene editing system in mice.Semantic proximity Read

Variants in Cas9 and <i>nanos</i> regulatory elements modulate activity and reduce resistance allele formation in homing gene driveSemantic proximity Read

Gene Editing and Gene Therapy: Entering Uncharted Territory in Veterinary OncologySemantic proximity Read

Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation.Semantic proximity Read

Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.Semantic proximity Read