Article

Endogenous intronic RNA tightly controls Cas9/CRISPR-mediated gene editing in human cells

2026-03-25

Abstract excerpt

<h4>ABSTRACT</h4> CRISPR/Cas approaches are often limited by off-target effects. In vivo, multiple cell types are often present and off-targets may result from unintended targeting of the wrong cells. In this work, we propose a solution to this limitation by using a transcribed intron of the target gene as an endogenous trigger (intron triggers) for a novel conditional guide RNA (intcgRNA). In vitro, intcgRNAs we...

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Literature Corpus work: edf78a35-33d1-5e1b-8e63-9a6eb6c362e5
DOI: 10.64898/2026.03.24.714022

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