Article

<i>In vitro</i> transcribed guide RNAs trigger an innate immune response via the RIG-I pathway

2018-03-03

Abstract excerpt

CRISPR-Cas9 genome editing is revolutionizing fundamental research and has great potential for the treatment of many diseases. While editing of immortalized cell lines has become relatively easy, editing of therapeutically relevant primary cells and tissues can remain challenging. One recent advancement is the delivery of a Cas9 protein and an in vitro transcribed (IVT) guide RNA (gRNA) as a precomplexed ribonucl...

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Literature Corpus work: 220c079f-e0d9-5439-ad8a-1465500c5912
DOI: 10.1101/275669

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