Article

Native ultrastructure of fresh human brain vitrified directly from autopsy revealed by cryo-electron tomography with cryo-plasma focused ion beam milling

2023-09-17

Abstract excerpt

Ultrastructure of human brain tissue has traditionally been examined using electron microscopy (EM) following chemical fixation, staining, and mechanical sectioning, which limit attainable resolution and introduce artifacts. Alternatively, cryo-electron tomography (cryo-ET) offers the potential to image unfixed cellular samples at higher resolution while preserving their native structures, but it requires samples...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: eb6a2a57-b245-508c-bda6-a42fb648526a
DOI: 10.1101/2023.09.13.557623

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Native ultrastructure of fresh human brain vitrified directly from autopsy revealed by cryo-electron tomography with cryo-plasma focused ion beam millingDOI 10.1101/2023.09.13.557623Read

Cryo-FIB workflow for imaging brain tissue via <i>in situ</i> cryo-electron microscopySemantic proximity Read

A robust workflow for 3D imaging of human mitochondria using cryo-electron tomographySemantic proximity Read

Serial cryoFIB/SEM Reveals Cytoarchitectural Disruptions in Leigh Syndrome Patient Cells.Semantic proximity Read

Volume electron microscopy reveals 3D synaptic nanoarchitecture in postmortem human prefrontal cortexSemantic proximity Read

Enabling FIB-SEM Systems for Large Volume Connectomics and Cell BiologySemantic proximity Read

Serial cryoFIB/SEM reveals profound cytoarchitectural disruptions caused by a pathogenic mutation in Leigh syndrome patient cellsSemantic proximity Read

Expansion Revealing of Pathology Resolves Nanostructures Associated with Inflammatory Phenotypes in COVID-19 Decedent Human Brain TissueSemantic proximity Read

Correlative light and electron microscopy for human brain and other biological modelsSemantic proximity Read

In Situ Architecture and Cellular Interactions of PolyQ Inclusions.Semantic proximity Read

Decrowding Expansion Pathology: Unmasking Previously Invisible Nanostructures and Cells in Intact Human Brain Pathology SpecimensSemantic proximity Read

Cryo-electron tomography of viral infection — from applications to biosafetySemantic proximity Read

Postmortem changes in brain cell structure: a reviewSemantic proximity Read