Article
Serial cryoFIB/SEM Reveals Cytoarchitectural Disruptions in Leigh Syndrome Patient Cells.
Structure (London, England : 1993) - 7 Jan 2021
Zhu Yanan, Sun Dapeng, Schertel Andreas, Ning Jiying, Fu Xiaofeng, Gwo Pam Pam, Watson Alan M, Zanetti-Domingues Laura C, Martin-Fernandez Marisa L, Freyberg Zachary, Zhang Peijun
Abstract excerpt
The advancement of serial cryoFIB/SEM offers an opportunity to study large volumes of near-native, fully hydrated frozen cells and tissues at voxel sizes of 10 nm and below. We explored this capability for pathologic characterization of vitrified human patient cells by developing and optimizing a serial cryoFIB/SEM volume imaging workflow. We demonstrate profound disruption of subcellular architecture in primary...
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