Article

Low-bias ncRNA Libraries using Ordered Two-Template Relay: Serial Template Jumping by a Modified Retroelement Reverse Transcriptase

2021-04-30

Abstract excerpt

Non-long terminal repeat (non-LTR) and group II intron retroelements encode reverse transcriptases (RTs) that copy the retroelement transcript directly into host cell DNA, often at specific target sites. Biochemical characterization of these enzymes has been limited by recombinant expression and purification challenges, hampering understanding of their transposition mechanism and their exploitation for research an...

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Literature Corpus work: ea2ebb4c-eaa3-5ea0-ace9-e5ef35011ef1
DOI: 10.1101/2021.04.30.442027

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