Article

qPCR Guru, a free browser-based platform, strengthens microRNA analyses using full-curve Cq estimation

2026-06-23

Abstract excerpt

Quantitative PCR (qPCR) depends on reliable quantification cycle (Cq) estimation from amplification curves, which are not always well-behaved. We developed qPCR Guru to provide a complete analysis pipeline including data quality assessment, relative quantification, standard-curve diagnostics, and dual-method Cq evaluation. The latter compares the conventional instrument-derived threshold (“Reported”) Cq versus the...

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Literature Corpus work: e8e5787c-0063-5a32-903b-4f5646813dac
DOI: 10.64898/2026.06.21.733609

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