Article
Quality control of digital PCR assays and platforms.
Analytical and bioanalytical chemistry - 1 Oct 2017
Vynck Matthijs, Vandesompele Jo, Thas Olivier
Abstract excerpt
Digital polymerase chain reaction (digital PCR, dPCR) is a direct nucleic acid quantification method, thus requiring no standard curves unlike quantitative real-time PCR (qPCR). Nevertheless, evaluation of the linear dynamic range, accuracy, and precision of an assay or platform is recommended, as there are several potential causes of important non-linearity, bias, and imprecision. Ignoring these quality issues...
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