Article

Evaluation validation of a qPCR curve analysis method and conventional approaches

2020-06-18

Abstract excerpt

Real-time quantitative polymerase chain reaction (qPCR) is a sensitive and reliable method for mRNA quantification and rapid analysis of gene expression from a large number of starting templates. It is based on the statistical significance of the beginning of exponential phase in real-time PCR kinetics, reflecting quantitative cycle of the initial target quantity and the efficiency of the PCR reaction (the fold in...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: e952d279-4b58-56bd-8bcb-85d898694b71
DOI: 10.1101/2020.06.18.158873

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Evaluation validation of a qPCR curve analysis method and conventional approachesDOI 10.1101/2020.06.18.158873Read

qPCR Guru, a free browser-based platform, strengthens microRNA analyses using full-curve Cq estimationSemantic proximity Read

A data-driven consensus framework for Ct interpretation in real-world multi-assay qPCR diagnosticsSemantic proximity Read

“Informative Use of Cycle-Threshold Values to Account for Sampling Variability in Pathogen Detection”Semantic proximity Read

Selection of appropriate reference genes for normalization of qRT-PCR based gene expression analysis in SARS-CoV-2, and Covid-associated Mucormycosis infectionSemantic proximity Read

Application of Pearson correlation coefficient (PCC) and Kolmogorov-Smirnov distance (KSD) metrics to identify disease-specific biomarker genesSemantic proximity Read

Normalization Strategy for Selection of Reference Genes for RT-qPCR Analysis in Left Ventricles of Failing Human HeartsSemantic proximity Read

gQuant: A Robust and Generalizable Algorithm for Identifying Normalizer Genes in qRT-PCR Data: A Case Study on Urinary Exosomal miRNAsSemantic proximity Read

Housekeeping Gene Expression Normalization in Transcriptomics Mitigates Data Leakage in Machine Learning ModelsSemantic proximity Read

Validation of Reference Genes for Oral Cancer Detection Panels in a Prospective Blinded Cohort.Semantic proximity Read

Selection of Ideal Reference Genes for Gene Expression Analysis in COVID-19 and MucormycosisSemantic proximity Read

Evaluation and validation of reference genes for RT-qPCR gene expression in Naegleria gruberiSemantic proximity Read

Auto-qPCR: A Python-based web app for automated and reproducible analysis of qPCR dataSemantic proximity Read