Article

Optimization of TaqMan-based quantitative PCR diagnosis for <i>Entamoeba histolytica</i> using Droplet Digital PCR

2025-02-25

Abstract excerpt

<h4>Background</h4> TaqMan-probed quantitative PCR (qPCR) is highly valued for diagnosing Entamoeba histolytica infections (amebiasis). However, unclear cycle threshold (Ct) values often yield low-titer positive results, complicating interpretation. This study aimed to optimize qPCR primer-probe sets with logically determined cut-off Ct value using droplet digital PCR (ddPCR). <h4>Methodology/Principal Findings...

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Literature Corpus work: 46dac2df-9786-583b-96ac-487285457235
DOI: 10.1101/2025.02.24.25322762

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