Article

Inexpensive and colorimetric RNA detection by <i>E. coli</i> cell-free protein synthesis platform at room temperature

2021-12-02

Abstract excerpt

<h4>ABSTRACT</h4> We report colorimetric detection of SARS-CoV-2 viral RNA by an in vitro transcription/translation assay with crude E. coli extracts at room temperature, with the aid of body heat. Clinically-relevant concentrations of viral RNA (ca. 600 copies/test) were detected from synthetic RNA samples. The activation of cell-free gene expression was achieved by toehold-switch-mediated riboregulatory elements...

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Literature Corpus work: e7bbd973-8dc9-5d0d-bbd2-740b098eda7f
DOI: 10.1101/2021.11.29.21267025

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