A liquid handling platform for standardised quantification of cell-free enzymatic activity encoded by antimicrobial resistance genes

Abstract excerpt

<h4>ABSTRACT</h4> Antimicrobial resistance (AMR) is a growing global threat to human health, and rapid methods for characterising emerging antimicrobial resistance genes (ARGs) are needed. Here, we develop a semi-automated workflow using cell-free gene expression (CFE) systems to measure the activity of two ARGs encoded on plasmid DNA that produce rifampicin-inactivating and gentamicin-inactivating enzymes. We va...

Topics

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• Anti-Bacterial Agents
• Bacterial Identification and Susceptibility Testing
• Drug Resistance, Bacterial
• Genotype
• Machine Learning
• Microbial Sensitivity Tests
• Phenotype
• RNA, Bacterial

Identifiers and source

Literature Corpus work

515fd42e-ec13-5c2f-8ceb-d8aa512b3c6a

DOI

10.64898/2026.04.23.720151