Article
Precise and Programmable Detection of Mutations Using Ultraspecific Riboregulators.
Cell - 5 Mar 2020
Hong Fan, Ma Duo, Wu Kaiyue, Mina Lida A, Luiten Rebecca C, Liu Yan, Yan Hao, Green Alexander A
Abstract excerpt
The ability to identify single-nucleotide mutations is critical for probing cell biology and for precise detection of disease. However, the small differences in hybridization energy provided by single-base changes makes identification of these mutations challenging in living cells and complex reaction environments. Here, we report a class of de novo-designed prokaryotic riboregulators that provide ultraspecific...
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