Article

Adaptive Sampling as tool for Nanopore direct RNA-sequencing

2022-10-18

Abstract excerpt

ONT long-read sequencing provides real-time monitoring and controlling of individual nanopores. Adaptive sampling enriches or depletes specific sequences in Nanopore DNA sequencing, but was not applicable to direct sequencing of RNA so far. Here, we identify essential parameter settings for direct RNA sequencing (DRS). We demonstrate the superior performance of depletion over enrichment and show that adaptive samp...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: e742db48-71b8-5a0d-994b-84ef1748e373
DOI: 10.1101/2022.10.14.512223

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Adaptive Sampling as tool for Nanopore direct RNA-sequencingDOI 10.1101/2022.10.14.512223Read

Poly(A) selection limits detection of long and alternatively spliced transcripts compared with rRNA depletion in RNA-SequencingSemantic proximity Read

Nanopore native RNA sequencing of a human poly(A) transcriptomeSemantic proximity Read

IVT-seq reveals extreme bias in RNA-sequencingSemantic proximity Read

Nanopore adaptive sequencing for mixed samples, whole exome capture and targeted panelsSemantic proximity Read

Adaptive nanopore sequencing for single cell characterization of cancer mutations and gene rearrangementsSemantic proximity Read

Multicellular, IVT-derived, unmodified human transcriptome for nanopore-direct RNA analysisSemantic proximity Read

RNA folding below the nanopore impairs direct RNA sequencing fidelity and modification callingSemantic proximity Read

A comprehensive examination of Nanopore native RNA sequencing for characterization of complex transcriptomesSemantic proximity Read

Improved long-transcript representation in Oxford Nanopore direct RNA sequencing with UltraMarathonRTSemantic proximity Read

ReadBouncer: Precise and Scalable Adaptive Sampling for Nanopore SequencingSemantic proximity Read

A high-efficient capture-based NGS approach for comprehensive analysis of mitochondrial transcriptomSemantic proximity Read

RNA modifications detection by comparative Nanopore direct RNA sequencingSemantic proximity Read