Article

Adaptive nanopore sequencing for single cell characterization of cancer mutations and gene rearrangements

2022-11-24

Abstract excerpt

<h4>ABSTRACT</h4> In this proof-of-concept study, we developed a single cell method that identifies somatic alterations found in coding regions of mRNAs and integrates these mutation genotypes with their matching cell transcriptomes. We used nanopore adaptive sampling on single cell cDNA libraries, generated long read sequences from target gene transcripts and identified coding variants among individual cells. Sh...

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Literature Corpus work: 0f2b3928-806e-54e1-8216-526c885641b0
DOI: 10.1101/2022.11.22.517284

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