Article

Engineering whole-cell biosensors to evaluate the effect of osmotic conditions on bacteria

2013-12-01

Abstract excerpt

Enhanced green fluorescent protein (eGFP) is a variant of wild-type GFP humanized for optimal expression in mammalian cell lines. A computational approach comparing wtGFP and eGFP showed the occurrence of rare proline codons within the eGFP gene that could interfere with and hamper protein production in prokaryotic expression systems. The eGFP gene excised from mammalian plasmid pEGFP N3 was used for construction...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: e53c7c62-8a59-5091-821e-5530816fe2ee
DOI: 10.1007/s13213-012-0587-4

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Engineering whole-cell biosensors to evaluate the effect of osmotic conditions on bacteriaDOI 10.1007/s13213-012-0587-4Read

Characterization of mutations affecting the osmoregulated proU promoter of Escherichia coli and identification of 5' sequences required for high-level expression.Semantic proximity Read

The Applications of Promoter-Gene Engineered Bio-SensorsSemantic proximity Read

Short, auto-inducible promoters for well-controlled protein expression in Escherichia coli.Semantic proximity Read

Expression of an engineered salt-inducible proline biosynthetic operon in a glutamic acid over-producing mutant, Halomonas elongata GOP, confers increased proline yield due to enhanced growth under high-salinity conditions.Semantic proximity Read

Effects of H-NS and potassium glutamate on sigmaS- and sigma70-directed transcription in vitro from osmotically regulated P1 and P2 promoters of proU in Escherichia coli.Semantic proximity Read

Engineering Extracellular Expression Systems in Escherichia coli Based on Transcriptome Analysis and Cell Growth State.Semantic proximity Read

Rational design of green fluorescent protein mutants as biosensor for bacterial endotoxin.Semantic proximity Read

Engineering and application of LacI mutants with stringent expressions.Semantic proximity Read

Design and characterization of auxotrophy-based amino acid biosensors.Semantic proximity Read

Screening of the osmotic pressure-inducible promoter regions from the whole genome of Escherichia coli by using a novel cloning method.Semantic proximity Read

Osmotic stress mechanically perturbs chemoreceptors in Escherichia coli.Semantic proximity Read

osmY, a new hyperosmotically inducible gene, encodes a periplasmic protein in Escherichia coli.Semantic proximity Read