Characterization of mutations affecting the osmoregulated proU promoter of Escherichia coli and identification of 5' sequences required for high-level expression.

doi:10.1128/jb.173.2.801-809.1991

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Article

Characterization of mutations affecting the osmoregulated proU promoter of Escherichia coli and identification of 5' sequences required for high-level expression.

Journal of bacteriology - 1 Jan 1991

Lucht J M, Bremer E

Abstract excerpt

Expression of the Escherichia coli proU operon, which encodes an efficient uptake system for the osmoprotectant glycine betaine, is strongly increased in cells grown at high osmolarity. We isolated 182 independent spontaneous mutants with elevated expression of the chromosomal phi(proV-lacZ) (Hyb...

Topics

Bacteriophage lambda
Base Sequence
Chromosome Deletion
Chromosomes, Bacterial
DNA Transposable Elements
Escherichia coli
Kinetics
Molecular Sequence Data
Mutation
Oligonucleotide Probes
Operon
Osmolar Concentration
Plasmids
Promoter Regions, Genetic
Recombinant Fusion Proteins
beta-Galactosidase

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Identifiers

PMID: 1846150
DOI: 10.1128/jb.173.2.801-809.1991
PMCID: PMC207074

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1846150-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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