Article
Effects of H-NS and potassium glutamate on sigmaS- and sigma70-directed transcription in vitro from osmotically regulated P1 and P2 promoters of proU in Escherichia coli.
Journal of bacteriology - 1 Jul 1996
Rajkumari K, Kusano S, Ishihama A, Mizuno T, Gowrishankar J
Abstract excerpt
We have used supercoiled DNA templates in this study to demonstrate that transcription in vitro from the P1 and P2 promoters of the osmoresponsive proU operon of Escherichia coli is preferentially mediated by the sigma(s) and sigma70-bearing RNA polymerase holoenzymes, respectively. Addition of p...
Topics
- Amino Acid Transport Systems
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Base Sequence
- Carrier Proteins
- Cell-Free System
- DNA, Superhelical
- DNA-Binding Proteins
- DNA-Directed RNA Polymerases
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Glutamates
- Molecular Sequence Data