Article
Screening of the osmotic pressure-inducible promoter regions from the whole genome of Escherichia coli by using a novel cloning method.
Biotechnology letters - 1 Apr 2008
Wang Zhijun, Jin Li, Wegrzyn Grzegorz, Wegrzyn Alicja
Abstract excerpt
Promoters belong to the most important regulatory domains in genomic sequences. A novel cloning method is described to produce various DNA fragments with the average size about 100 bp, which differ by 1 bp, for screening of the osmotic pressure-inducible promoters from the genome of Escherichia coli. An osmotic pressure-inducible promoter sequence was found. A mutation in this promoter sequence, introduced by...
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