Article

An optimised method for generation of murine CAR-T cells by CRISPR/Cas9

2025-11-29

Abstract excerpt

Development of the next generation of chimeric antigen receptor (CAR) T-cells requires assessment in systems that better recapitulate the suppressive tumour microenvironment of solid tumours. CRISPR-Cas9 knock-in of promoter-less homology directed repair templates (HDRT) into the T-cell receptor locus has been shown to result in physiological expression of CARs with improved tumour control. We initially compared t...

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Literature Corpus work: e4d03709-b2be-54f2-a91c-64ab4a343c0a
DOI: 10.1101/2025.11.29.689298

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