Article

SIRVs: Spike-In RNA Variants as External Isoform Controls in RNA-Sequencing

2016-10-13

Abstract excerpt

Spike-In RNA variants (SIRVs) enable for the first time the validation of RNA sequencing workflows using external isoform transcript controls. 69 transcripts, derived from seven human model genes, cover the eukaryotic transcriptome complexity of start- and end-site variations, alternative splicing, overlapping genes, and antisense transcription in a condensed format. Reference RNA samples were spiked with SIRV mix...

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Literature Corpus work: e33596ee-3cf3-55f0-8618-1be695b4ce94
DOI: 10.1101/080747

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