Article

Synthesizing human-specific spike-in standards for RNA-seq experiments and assessing their technical performance

2025-04-07

Abstract excerpt

Using spike-in standards for RNA-seq experiments is critical to evaluate technical bias introduced during sample preparation, sequencing and analysis. Although some external RNA spike-in standards have been developed, species-specific spike-in standard was not reported yet. Here we developed the human-specific spike-in standards with 65 controls. We first extracted human endogenous RNAs with various lengths and GC...

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Literature Corpus work: 64191023-d8ca-50d0-8e66-942ff77edf87
DOI: 10.1101/2025.04.01.646725

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