Article

Evaluating Methods for Differential Gene Expression And Alternative Splicing Using Internal Synthetic Controls

2020-08-06

Abstract excerpt

High-throughput transcriptome sequencing has become a powerful tool in the study of human diseases. Identification of causal mechanisms may entail analysis of differential gene expression (DGE), differential transcript/isoform expression (DTE) and identification, classification and quantification of alternative splicing (AS) and/or detection of novel AS events. For such a global transcriptome profiling execution o...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 636165da-6f13-56d0-844f-5c7e83e7c572
DOI: 10.1101/2020.08.05.238295

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Evaluating Methods for Differential Gene Expression And Alternative Splicing Using Internal Synthetic ControlsDOI 10.1101/2020.08.05.238295Read

SIRVs: Spike-In RNA Variants as External Isoform Controls in RNA-SequencingSemantic proximity Read

Transcriptome Universal Single-isoform COntrol: A Framework for Evaluating Transcriptome reconstruction QualitySemantic proximity Read

Selecting differential splicing methods: Practical considerationsSemantic proximity Read

Selecting differential splicing methods: Practical considerations for short-read RNA sequencingSemantic proximity Read

Foreign RNA spike-ins enable accurate allele-specific expression analysis at scaleSemantic proximity Read

Alternative splicing detection workflow needs a careful combination of sample prep and bioinformatics analysisSemantic proximity Read

Synthesizing human-specific spike-in standards for RNA-seq experiments and assessing their technical performanceSemantic proximity Read

<i>SplAdder</i> : Identification, quantification and testing of alternative splicing events from RNA-Seq dataSemantic proximity Read

Foreign RNA spike-ins enable accurate allele-specific expression analysis at scale.Semantic proximity Read

A benchmarking of workflows for detecting differential splicing and differential expression at isoform level in human RNA-seq studiesSemantic proximity Read

RNA sequencing depth guidelines for the study of alternative splicingSemantic proximity Read

Toward Best Practice in Identifying Subtle Differential Expression with RNA-seq: A Real-World Multi-Center Benchmarking Study Using Quartet and MAQC Reference MaterialsSemantic proximity Read