Article

Comparative Analysis of Single-Nucleus and Single-Cell RNA Sequencing in Human Bone Marrow Mononuclear Cells: Methodological Insights and Trade-offs

2025-09-09

Abstract excerpt

Bone marrow mononuclear cells (BMMCs) are a heterogeneous pool of hematopoietic progenitors and mature immune cells that collectively sustain hematopoiesis and coordinate immune responses. The bone marrow serves not only as the primary site for blood cell production but also as a niche for various disorders, including blood cancers. Advances in single-cell RNA sequencing (scRNA-seq) and single-nucleus RNA sequenci...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 911fd50a-a0c9-5f34-bf9f-1648fc271eac
DOI: 10.1101/2025.09.08.675012

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Comparative Analysis of Single-Nucleus and Single-Cell RNA Sequencing in Human Bone Marrow Mononuclear Cells: Methodological Insights and Trade-offsDOI 10.1101/2025.09.08.675012Read

Technical Advances in Single-Cell RNA Sequencing and Applications in Normal and Malignant HematopoiesisSemantic proximity Read

Single‐Cell RNA Sequencing in Hematological DiseasesSemantic proximity Read

Comparative analysis of single-cell RNA sequencing methods with and without sample multiplexingSemantic proximity Read

Current Methodological Challenges of Single-Cell and Single-Nucleus RNA-Sequencing in Glomerular DiseasesSemantic proximity Read

Benchmarking Single-Cell RNA Sequencing Protocols for Cell Atlas ProjectsSemantic proximity Read

Evaluation of single-cell RNA profiling technologies using FFPE, fresh, and frozen ccRCC tumor specimensSemantic proximity Read

Single-Nucleus RNA-Seq: Open the Era of Great Navigation for FFPE TissueSemantic proximity Read

Single cell RNA sequencing improves the next generation of approaches to AML treatment: challenges and perspectivesSemantic proximity Read

A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumorsSemantic proximity Read

Characterization of The Heterogeneity of Bone Marrow in NK-AML (M4/M5) Based on Single Cell RNA SequencingSemantic proximity Read

Sample-multiplexed FACS-preprocessing of PBMCs enables scalable scRNA-seq without compromising transcriptomic or cellular integritySemantic proximity Read

An optimized workflow for single-cell transcriptomics and repertoire profiling of purified lymphocytes from clinical samplesSemantic proximity Read