Article

Q-Bodies from Recombinant Single-Chain Fv Fragment with Better Yield and Expanded Palette of Fluorophores

2015-10-26

Abstract excerpt

Fluorescence-based immunosensors serve a vital role in biotechnology and diagnostic and therapeutic applications. Our group recently developed a unique fluoroimmunosensor named Quenchbody (Q-body) that operates based on the principle of quenching and the antigen-dependent release of fluorophore, which is incorporated to a recombinant antibody fragment, either the single-chain Fv (scFv) or the Fab fragment of an an...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: e09364a3-2937-5336-bb59-8342bf24fecb
DOI: 10.1021/acssensors.5b00089

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Q-Bodies from Recombinant Single-Chain Fv Fragment with Better Yield and Expanded Palette of FluorophoresDOI 10.1021/acssensors.5b00089Read

Development of a Functional Antibody by Using a Green Fluorescent Protein Frame as the TemplateSemantic proximity Read

Evaluation and Selection of Potent Fluorescent Immunosensors by Combining Fluorescent Peptide and Nanobodies Displayed on Yeast SurfaceSemantic proximity Read

Single-chain fragment variable produced by phage display technology: Construction, selection, mutation, expression, and recent applications in food safety.Semantic proximity Read

Reporter–nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagentsSemantic proximity Read

Solubility, Stability, and Avidity of Recombinant Antibody Fragments Expressed in MicroorganismsSemantic proximity Read

Optimised nanobody-based quenchbodies for enhanced protein detectionSemantic proximity Read

Protein engineering of antibody fragments for pharmaceutical productionSemantic proximity Read

Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodiesSemantic proximity Read

A novel efficient bispecific antibody format, combining a conventional antigen-binding fragment with a single domain antibody, avoids potential heavy-light chain mis-pairing.Semantic proximity Read

Engineered Bifunctional Proteins for Targeted Cancer Therapy: Prospects and ChallengesSemantic proximity Read

Design of an alternate antibody fragment format that can be produced in the cytoplasm of Escherichia coliSemantic proximity Read

Efficient expression of full-length antibodies in the cytoplasm of engineered bacteriaSemantic proximity Read