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An optimized circular polymerase extension reaction-based method for functional analysis of SARS-CoV-2

7 Apr 2023

Abstract excerpt

BACKGROUND: Reverse genetics systems have been crucial for studying specific viral genes and their relevance in the virus lifecycle, and become important tools for the rational attenuation of viruses and thereby for vaccine design. Recent rapid progress has been made in the establishment of reverse genetics systems for functional analysis of SARS-CoV-2, a coronavirus that causes the ongoing COVID-19 pandemic that...

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PMID: 37029393
DOI: 10.1186/s12985-023-02025-y

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