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Rapid nanopore sequencing and predictive susceptibility testing of positive blood cultures from intensive care patients with sepsis

9 Jan 2024

Abstract excerpt

ABSTRACT We aimed to evaluate the performance of Oxford Nanopore Technologies (ONT) sequencing from positive blood culture (BC) broths for bacterial identification and antimicrobial susceptibility prediction. Patients with suspected sepsis in four intensive care units were prospectively enrolled. Human-depleted DNA was extracted from positive BC broths and sequenced using ONT (MinION). Species abundance was...

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PMID: 38193658
DOI: 10.1128/spectrum.03065-23

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