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PathwayBench: a multi-criterion benchmark of pseudobulk pathway activity scoring methods reveals rank-window competition as a mechanism of biological signal loss in single-cell RNA-seq

2026-07-10

Abstract excerpt

<title>Abstract</title> <p>Background Pathway activity scoring is a foundational step in single-cell RNA-seq (scRNA-seq) analysis, yet method choice is rarely guided by systematic, multi-criterion evidence in the pseudobulk case-control regime that now dominates applied single-cell disease studies. Prior single-cell pathway-scoring benchmarks have focused on perturbation ground truth at cell-level resolution, le...

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Literature Corpus work: db81d0d1-041c-55d8-bd8b-25d6b3c1ae28
DOI: 10.21203/rs.3.rs-10271634/v1

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PathwayBench: a multi-criterion benchmark of pseudobulk pathway activity scoring methods reveals rank-window competition as a mechanism of biological signal loss in single-cell RNA-seqDOI 10.21203/rs.3.rs-10271634/v1Read

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