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Article

Massively parallel single-cell B-cell receptor sequencing enables rapid discovery of diverse antigen-reactive antibodies

2019-06-06

Abstract excerpt

<h4>ABSTRACT</h4> Using high-throughput single-cell B-cell receptor sequencing (scBCR-seq) we obtained accurately paired full-length heavy- and light-chain variable domains from thousands of individual B cells in a massively parallel fashion. We sequenced more than 250,000 B cells from rat, mouse and human repertoires to characterize their lineages and expansion. In addition, we immunized rats with chicken ovalbu...

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Literature Corpus work
db5b2e52-99ed-58b9-acc0-146fdac61373
DOI
10.1101/660472
Open publication

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