Multiplexed antigen panel analysis identifies B cell phenotype and receptor genetic contributions to antibody breadth.

doi:10.1016/j.immuni.2026.03.006

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Article

Multiplexed antigen panel analysis identifies B cell phenotype and receptor genetic contributions to antibody breadth.

Immunity - 12 May 2026

Wirz Oliver F, Kotagiri Prasanti, Haraguchi Emily, Röltgen Katharina, Hunter Molly, Craig Erin, Afaghani Jumana, Lee Ji-Yeun, Nielsen Sandra C A, Hoh Ramona A, Pursell Taylor, Arunachalam Prabhu S, Manohar Monali, Chang Iris, Schuetz Jackson P, Lam Brandon, Fernandes Andrea, Do Evan, Smith Donna, Ha Brian, Liao Linda, Najeeb Javaria, Otrelo-Cardoso Ana R, Ho Chandler, Wohlstadter Jacob N, Sigal George B, Jardetzky Theodore S, Scott Stuart A, Van Slyck Sean, Davis Mark M, Pulendran Bali, Minshull Jeremy, Pinsky Benjamin A, Nadeau Kari C, Niemann Claus U, Yang Fan, Boyd Scott D

Abstract excerpt

The cellular, immunogenetic, and antigenic factors affecting the breadth of viral antigen variants recognized by human antibody responses are poorly defined. We developed highly multiplexed panels of DNA-tagged SARS-CoV-2 antigens from up to 20 viral variants to label and sort 6,262 antigen-binding circulating B cells from previously naive mRNA vaccinees or infected patients, and from deceased organ donor...

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Identifiers

PMID: 41928519
DOI: 10.1016/j.immuni.2026.03.006
PMCID: PMC13456080

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

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