Article

Fluorescence cross-correlation spectroscopy quantifies affinity, cooperativity, and kinetic stability in ternary protein complexes

2026-07-17

Abstract excerpt

Many biological processes and emerging therapeutic modalities rely on higher-order protein complexes whose properties cannot be predicted from their constituent binary interactions. However, methods for directly quantifying affinity, cooperativity, and kinetic stability within such assemblies remain limited. Here, we establish fluorescence cross-correlation spectroscopy (FCCS) as a solution-phase approach for char...

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Literature Corpus work: daccf43c-724d-5080-895b-d5074f2771d8
DOI: 10.64898/2026.07.16.738902

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