Article

Direct RNA sequencing of primary human T cells reveals the impact of immortalization on mRNA pseudouridine modifications

2025-03-10

Abstract excerpt

Immortalized cell lines are commonly used as proxies for primary cells in human biology research. For example, Jurkat leukemic T cells fundamentally contributed to uncovering T cell signaling, activation, and immune responses. However, the immortalization process can alter key cellular properties, and researchers widely believe this process could significantly change RNA modification machinery and modification sit...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: d7c27871-dd9b-542e-809d-67a9f13b21ab
DOI: 10.1101/2025.03.02.641090

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Direct RNA sequencing of primary human T cells reveals the impact of immortalization on mRNA pseudouridine modificationsDOI 10.1101/2025.03.02.641090Read

Multicellular, IVT-derived, unmodified human transcriptome for nanopore-direct RNA analysisSemantic proximity Read

Semi-quantitative detection of pseudouridine modifications and type I/II hypermodifications in human mRNAs using direct and long-read sequencingSemantic proximity Read

Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolutionSemantic proximity Read

The RNA Atlas, a single nucleotide resolution map of the human transcriptomeSemantic proximity Read

Quantitative profiling of pseudouridylation landscape in the human transcriptomeSemantic proximity Read

Recent developments, opportunities, and challenges in the study of mRNA pseudouridylationSemantic proximity Read

Messenger-RNA Modification Standards and Machine Learning Models Facilitate Absolute Site-Specific Pseudouridine QuantificationSemantic proximity Read

Exploring pseudouridylation: dysregulation in disease and therapeutic potentialSemantic proximity Read

Pseudouridine synthases modify human pre-mRNA co-transcriptionally and affect splicingSemantic proximity Read

Tabula Sapiens reveals the non-coding RNA landscape across 22 human organs and tissuesSemantic proximity Read

Epitranscriptomics as a New Layer of Regulation of Gene Expression in Skeletal Muscle: Known Functions and Future PerspectivesSemantic proximity Read

Direct RNA nanopore sequencing reveals rapid RNA modification changes following glucose stimulation of human pancreatic beta-cell linesSemantic proximity Read