Article

Time- and Cost-effective Production of Untagged Recombinant Mva by Flow Virometry and Direct Virus Sorting

2023-06-28

Abstract excerpt

<title>Abstract</title><p>Background Recombinant MVAs (rMVAs) are widely used both in basic and clinical research. Our previously developed Red-to-Green Gene Swapping Method (RGGSM), a cytometry-based Cell-Sorting protocol, revolves around the transient expression of a green fluorescent cytoplasmic marker, to subsequently obtain purified untagged rMVA upon loss of that marker by site-specific recombination. The st...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: d60626af-dcc8-5f34-9b99-6f9deab64454
DOI: 10.21203/rs.3.rs-3071499/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Time- and Cost-effective Production of Untagged Recombinant Mva by Flow Virometry and Direct Virus SortingDOI 10.21203/rs.3.rs-3071499/v1Read

Single-Particle Discrimination of Retroviruses from Extracellular Vesicles by Nanoscale Flow Cytometry.Semantic proximity Read

Multi-color fluorescent reporter dengue viruses with improved stability for analysis of a multi-virus infectionSemantic proximity Read

Coronavirus membrane protein with a fluorescent protein tag enables tracking of virus particles in live cellsSemantic proximity Read

Distinguishing Pseudotransduction and True Transduction Enables Characterization and Bioengineering of Extracellular Vesicle-Adeno-Associated Virus VectorsSemantic proximity Read

Engineered CHO cells as a novel AAV production platform for gene therapy deliverySemantic proximity Read

NEO-AAV: an engineered extracellular vesicle-enveloped AAV platform for activation-coupled T cell transduction and CAR-T cell generationSemantic proximity Read

Faster Replication and Higher Expression Levels of Viral Glycoproteins Give the Vesicular Stomatitis Virus/Measles Virus Hybrid VSV-FH a Growth Advantage over Measles VirusSemantic proximity Read

A reporter cell line for the automated quantification of SARS-CoV-2 infection in living cellsSemantic proximity Read

Reporters of gene expression: autofluorescent proteins.Semantic proximity Read

Detection of tumor-derived extracellular vesicles interactions with immune cells is dependent on EV-labelling methodsSemantic proximity Read

Non-permissive CHO cells; A rapid approach for purification of recombinant Herpes Simplex Virus-1Semantic proximity Read

Snorkel-tag Based Affinity Chromatography for Recombinant Extracellular Vesicle PurificationSemantic proximity Read