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Multi-color fluorescent reporter dengue viruses with improved stability for analysis of a multi-virus infection

16 Mar 2018

Abstract excerpt

Reporter virus is a versatile tool to visualize and to analyze virus infections. However, for flaviviruses, it is difficult to maintain the inserted reporter genes on the viral genome, limiting its use in several studies that require homogeneous virus particles and several rounds of virus replication. Here, we showed that flanking inserted GFP genes on both sides with ribosome-skipping 2A sequences improved the...

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PMID: 29547653
DOI: 10.1371/journal.pone.0194399

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