Article

Protocol to isolate oligodendrocytes, microglia, endothelial cells, astrocytes, and neurons from a single mouse brain using magnetic-activated cell sorting

2025-08-12

Abstract excerpt

<h4>Summary</h4> The isolation of specific cell types of the brain is essential to study cell-type-specific differences in complex neurological diseases such as Alzheimer’s disease. This protocol isolates oligodendrocytes, microglia, endothelial cells, astrocytes, and neurons from a single mouse brain. The process involves gentle tissue homogenization, debris removal, and sequential sorting of five distinct cell...

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Literature Corpus work: d3bcaf55-4252-5195-9f80-295d970d3105
DOI: 10.1101/2025.08.08.666877

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