High yield extraction of pure spinal motor neurons, astrocytes and microglia from single embryo and adult mouse spinal cord.

doi:10.1038/srep16763

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Article

High yield extraction of pure spinal motor neurons, astrocytes and microglia from single embryo and adult mouse spinal cord.

Scientific reports - 18 Nov 2015

Beaudet Marie-Josée, Yang Qiurui, Cadau Sébastien, Blais Mathieu, Bellenfant Sabrina, Gros-Louis François, Berthod François

Abstract excerpt

Extraction of mouse spinal motor neurons from transgenic mouse embryos recapitulating some aspects of neurodegenerative diseases like amyotrophic lateral sclerosis has met with limited success. Furthermore, extraction and long-term culture of adult mouse spinal motor neurons and glia remain also challenging. We present here a protocol designed to extract and purify high yields of motor neurons and glia from...

Topics

Animals
Astrocytes
Biomarkers
Cell Separation
Cell Survival
Cells, Cultured
Embryo, Mammalian
Gene Expression
Mice
Mice, Transgenic
Microglia
Motor Neurons
Phenotype
Spinal Cord

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Identifiers

PMID: 26577180
DOI: 10.1038/srep16763
PMCID: PMC4649473

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