Article
Methodological comparison of FACS and MACS isolation of enriched microglia and astrocytes from mouse brain.
Journal of immunological methods - 1 Nov 2020
Pan Jie, Wan Jun
Abstract excerpt
Microglia and astrocytes, the two innate cells in CNS, are thought to protect and remodel of synapses for proper maintenance and plasticity of neuronal circuits. The two types of cells are the major responders by producing and releasing inflammatory mediators. Isolation of microglia and astrocytes from CNS tissue provides a powerful tool to study basic cell biology and examine the effects of in vivo treatments on...
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