Article

<i>dotdotdot</i> : an automated approach to quantify multiplex single molecule fluorescent in situ hybridization (smFISH) images in complex tissues

2019-09-25

Abstract excerpt

<h4>ABSTRACT</h4> Multiplex single-molecule fluorescent in situ hybridization (smFISH) is a powerful method for validating RNA sequencing and emerging spatial transcriptomic data, but quantification remains a computational challenge. We present a framework for generating and analyzing smFISH data in complex tissues while overcoming autofluorescence and increasing multiplexing capacity. We developed dotdotdot (...

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Literature Corpus work: ce7bf173-c3d5-5f47-9285-2038ea63f7c4
DOI: 10.1101/781559

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