Article

Contamination as a major factor in poor Illumina assembly of microbial isolate genomes

2016-10-19

Abstract excerpt

<h4>ABSTRACT</h4> The nonhybrid hierarchical assembly of PacBio long reads is becoming the most preferred method for obtaining genomes for microbial isolates. On the other hand, among massive numbers of Illumina sequencing reads produced, there is a slim chance of re-evaluating failed microbial genome assembly (high contig number, large total contig size, and/or the presence of low-depth contigs). We generated Il...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: c9697bd7-d76f-58e0-9a91-442a35a8bcf0
DOI: 10.1101/081885

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Contamination as a major factor in poor Illumina assembly of microbial isolate genomesDOI 10.1101/081885Read

Contaminant DNA in bacterial sequencing experiments is a major source of false genetic variability.Semantic proximity Read

Benchmarking <i>de novo</i> assembly methods on metagenomic sequencing dataSemantic proximity Read

The Human “Contaminome”: Bacterial, Viral, and Computational Contamination in Whole Genome Sequences from 1,000 FamiliesSemantic proximity Read

Contaminant DNA in bacterial sequencing experiments is a major source of false genetic variabilitySemantic proximity Read

Diverse and widespread contamination evident in the unmapped depths of high throughput sequencing dataSemantic proximity Read

Small amounts of misassembly can have disproportionate effects on pangenome-based metagenomic analysesSemantic proximity Read

A Comprehensive Investigation of Metagenome Assembly by Linked-Read SequencingSemantic proximity Read

From trash to treasure: detecting unexpected contamination in unmapped NGS dataSemantic proximity Read

Understanding trivial challenges of microbial genomics: An assembly exampleSemantic proximity Read

Semi-automatic in silico gap closure enabled de novo assembly of two Dehalobacter genomes from metagenomic data.Semantic proximity Read

Genome complexity of harmful microalgae.Semantic proximity Read

Amplification-free Illumina sequencing-library preparation facilitates improved mapping and assembly of (G+C)-biased genomes.Semantic proximity Read