Article

Quantitative fluorescence lifetime imaging uncovers a novel role for KCC2 chloride transport in dendritic microdomains

2020-06-09

Abstract excerpt

Intracellular chloride ion ([Cl − ] i ) homeostasis is critical for synaptic neurotransmission yet variations in subcellular domains are poorly understood owing to difficulties in obtaining quantitative, high-resolution measurements of dendritic [Cl − ] i . We combined whole-cell patch clamp electrophysiology with simultaneous fluorescence lifetime imaging (FLIM) of the Cl − dye MQAE to quantitatively map den...

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Literature Corpus work: c8b424b1-93ce-5924-8145-96c74cc79ad7
DOI: 10.1101/2020.06.08.139592

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