Article

Targeted transcript quantification in single disseminated cancer cells after whole transcriptome amplification

2019-04-23

Abstract excerpt

Gene expression analysis of rare or heterogeneous cell populations such as disseminated cancer cells (DCCs) requires a sensitive method allowing reliable analysis of single cells. Therefore, we developed and explored the feasibility of a quantitative PCR (qPCR) assay to analyze single-cell cDNA pre-amplified using a previously established whole transcriptome amplification (WTA) protocol. We carefully selected and...

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Literature Corpus work: c6ca325e-c7f4-5b97-8a1a-9fe1c382b8f1
DOI: 10.1101/616839

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