Article
Poly (A)+ transcriptome assessment of ERBB2-induced alterations in breast cell lines.
PloS one - 1 Jan 2011
Carraro Dirce Maria, Ferreira Elisa Napolitano, de Campos Molina Gustavo, Puga Renato David, Abrantes Eduardo Fernandes, Trapé Adriana Priscila, Eckhardt Bedrich L, Ekhardt Bedrich L, Nunes Diana Noronha, Brentani Maria Mitzi, Arap Wadih, Pasqualini Renata, Brentani Helena, Dias-Neto Emmanuel, Brentani Ricardo Renzo
Abstract excerpt
We report the first quantitative and qualitative analysis of the poly (A)⁺ transcriptome of two human mammary cell lines, differentially expressing (human epidermal growth factor receptor) an oncogene over-expressed in approximately 25% of human breast tumors. Full-length cDNA populations from the two cell lines were digested enzymatically, individually tagged according to a customized method for library...
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