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Article

Implementation of the CRISPR-Cas9 system in fission yeast.

Nature communications - 29 Oct 2014

Jacobs Jake Z, Ciccaglione Keith M, Tournier Vincent, Zaratiegui Mikel

Abstract excerpt

Application of the CRISPR-Cas9 genome editing system in the model organism Schizosaccharomyces pombe has been hampered by the lack of constructs to express RNA of arbitrary sequence. Here we present expression constructs that use the promoter/leader RNA of K RNA (rrk1) and a ribozyme to produce t...

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Identifiers

PMID: 25352017
DOI: 10.1038/ncomms6344
PMCID: PMC4215166

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-25352017-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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