Article

RNA-Seq is not required to determine stable reference genes for qPCR normalization

2021-08-23

Abstract excerpt

Assessment of differential gene expression by qPCR is heavily influenced by the choice of reference genes. Although numerous statistical approaches have been proposed to determine the best reference genes, they can give rise to conflicting results depending on experimental conditions. Hence, recent studies propose the use of RNA-Seq to identify stable genes followed by the application of different statistical appr...

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Literature Corpus work: c3292282-1804-5178-aba1-812347bc2776
DOI: 10.1101/2021.08.21.457202

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