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Article

Identification of new reference genes for the normalisation of canine osteoarthritic joint tissue transcripts from microarray data

1 Jan 2007

Abstract excerpt

BACKGROUND: Real-time reverse transcriptase quantitative polymerase chain reaction (real-time RT-qPCR) is the most accurate measure of gene expression in biological systems. The comparison of different samples requires the transformation of data through a process called normalisation. Reference or housekeeping genes are candidate genes which are selected on the basis of constitutive expression across samples, and...

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PMID: 17651481
DOI: 10.1186/1471-2199-8-62

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